Enhanced Resolution in the Fingerprinting of Short-Chain Oligonucleotides Using the Dodecasodium Salt of Phytic Acid in Capillary Electrophoresis

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Abstract
The inclusion of the dodecasodium salt of phytic acid in separation buffers has been found to improve considerably the separation of a number of synthetic short-chain oligonucleotides. A combination of the simplicity and the improved resolving power of this methodology allows the facile analysis of mixtures of such compounds. The presence of a large concentration of sodium ions reduces electroosmotic mobility due to more efficient shielding of negative charges on the inner surface of the capillary wall. This, in turn, leads to a more gradual fall in potential between this surface and the center of the capillary.