The effect of hyposmotic and sodium chloride hyperosmotic environments on the secretion and synthesis of prolactin from human decidua in vitro

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Abstract
Using a paired design and a cross-over design both in 16 h incubation periods, human decidua were incubated in hyposmotic, isosmotic and NaCl hyperosmotic media. In the cross-over design each tissue aliquot was subjected to 3 media with different osmolalities, one of which was isosmotic. Thus, intensive utilization of the tissue was possible. The results of the cross-over study were identical with the result of the paired design. A decreased secretion of prolactin in hyposmotic environments, was found whereas hyperosmotic environments up to an osmolality .apprx. 400 mmol/kg gave a dose related increase in the prolactin secretion into the medium and in the total content of prolactin in the tissue and the medium. Excessive hyperosmotic medium decreased the secretion. The enhanced secretion was an effect of increased production, which could be blocked by cycloheximide. No feedback of prolactin on its own secretion was found.