Influence of Tissue Culture Passage, Storage, Temperature and Drying on Viability of S E polyoma Virus

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Abstract
The SE polyoma virus retained its capacity to induce tumors in hamsters after more than 35 continuous tissue culture passages; after storage at -70[degree]C for 404 days, at -20[degree]C for 329 days, and at 4[degree]C for 169 days; lyophilization; and treatment with ether at room temperature for 14 days, with trypsin at 36[degree]C for 18 hours or with 33% glycerol at 4[degree]C for 72 days. The virus was not destroyed by heating to 60[degree]C for 30 minutes, but 70[degree]C for 30 minutes destroyed or inhibited the property of tumor induction as evidenced by development of tumors in only 4 of 18 injected hamsters.

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