Physicochemical Characterization of A and B Subunits of Shiga Toxin and Reconstitution of Holotoxin from Isolated Subunits
- 1 March 1987
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 31 (3) , 189-197
- https://doi.org/10.1111/j.1348-0421.1987.tb03083.x
Abstract
The A and B subunits of Shiga toxin were isolated by high performance liquid chromatography and their physicochemical properties were examined. The A subunit of Shiga toxin purified from culture supernatant was not nicked, but it could be nicked in vitro by trypsin. The isoelectric points of the A and B subunits were determined to be 8.2 and 5.8, respectively. Amino acid compositions of the two subunits were also determined. The isolated A and B subunits were reconstituted to form active holotoxin which showed lethal activity to mice which was similar to that of native Shiga toxin.This publication has 19 references indexed in Scilit:
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