Electron immunocytochemical localization of Actinobacillus actinomycetemcomitans leukotoxin
- 1 February 1992
- journal article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 7 (1) , 24-27
- https://doi.org/10.1111/j.1399-302x.1992.tb00015.x
Abstract
The Actinobacillus actinomycetemcomitans leukotoxin was localized in A. actinomycetemcomitans bacteria using an electron immunocytochemical thin-section labeling method. An immuno-dot blot procedure was initially used to ascertain the optimal specimen fixation. This consisted of a periodate-lysine-paraformaldehyde (2%) fixative in a phosphate buffer followed by embedding in LR White. Affinity-purified toxin was used to produce a monospecific polyclonal antibody. The reaction sites were visualized with a colloidal gold-tagged reporter antibody. The leukotoxin was found to be localized either in the cell envelope and/or in membranous vesicles on the outer surface of the bacterial cell. These results support previous observations indicating the same location.Keywords
This publication has 31 references indexed in Scilit:
- Identification and expression of the Actinobacillus actinomycetemcomitans leukotoxin geneBiochemical and Biophysical Research Communications, 1989
- Distribution of Actinobacillus actinomycetemcomitans in localized juvenile periodontitis plaque: an electron immunocytochemical studyJournal of Periodontal Research, 1986
- Characterisation of HlyC and mechanism of activation and secretion of haemolysin from E. coli 2001FEBS Letters, 1985
- A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscopeJournal of Molecular Histology, 1983
- THE PRESERVATION OF ULTRASTRUCTURE AND ANTIGENICITYJournal of Microscopy, 1982
- Electron immunoperoxidase staining in sections of unfixed pure cultures ofStreptococcus mutansActa Odontologica Scandinavica, 1981
- Comparative ultrastructure of leukotoxic and non‐leukotoxic strains of Actinobacillus actinomycetemcomitansJournal of Periodontal Research, 1981
- Actinobacillus actinomycetemcomitans endocarditis: report of a case and review of the literatureThe Lancet Healthy Longevity, 1973
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966
- Fixation of the central nervous system for electron microscopy by aldehyde perfusionJournal of Ultrastructure Research, 1965