IMMUNOLOGICAL BLOCK TO SYNTHETIC ALPHA-MELANOCYTE-STIMULATING HORMONE - MELANOCYTE INTERACTION BY ANTIBODIES ISOLATED FROM CELL-COLUMN IMMUNOADSORBENTS
- 1 January 1979
- journal article
- research article
- Vol. 39 (8) , 3058-3064
Abstract
Antibodies to formalin-fixed, syngeneic melanoma cells were prepared in mice, purified by immunoaffinity chromatography and tested for binding activity to viable melanoma cells. The radiolabeled antibodies detected .simeq. 9 .times. 106 melanoma antigenic sites/cell. The calculated average association constant (Ka) for the antibody population was 7-10 .times. 107 M-1. The antibody blocked the binding of melanocyte-stimulating hormone in competitive cell surface binding studies. Results are discussed conceptually in terms of the potentially important role that the humoral immune response may play in the phenomenon of tumor progression.This publication has 15 references indexed in Scilit:
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