IMMUNOPOTENTIATION OF THE HUMORAL RESPONSE BY LIPOSOMES - ENCAPSULATION VERSUS COVALENT LINKAGE
- 1 October 1988
- journal article
- research article
- Vol. 65 (2) , 315-317
Abstract
Two different modes of antigen association with liposomes were compared for their stimulation of IgM- and IgG-producing cells in primary- and secondary-response experiments. The study was carried out on BALB/c mice using the antigen bovine serum albumin either free, encapsulated in liposomes or covalently linked to the liposomal surface. Our results indicate that, although both types of liposome association are equally efficient in potentiating the humoral response, encapsulation mainly favours IgG isotype production with little or no effect on the IgM subset, while covalent linkage stimulates the production of both IgG and IgM. Our results reconcile some apparently conflicting published data and suggest that the mode of antigen association with liposomes considerably influences the pathways by which stimulation occurs.This publication has 18 references indexed in Scilit:
- MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED AND UNRESTRICTED ACTIVATION OF HELPER T-CELL LINES BY LIPOSOME-BOUND ANTIGENS1986
- Association of an albumin antigen with phosphatidylcholine liposomes alters the nature of immunoglobulins produced during the immune response against the antigenBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- A New Approach for the Immunogenic Presentation of Membrane-Bound Human Colon Tumor AntigensImmunological Communications, 1982
- IMMUNE-RESPONSE MEDIATED BY LIPOSOME-ASSOCIATED PROTEIN ANTIGENS .2. COMPARISON OF THE EFFECTIVENESS OF VESICLE-ENTRAPPED AND SURFACE-ASSOCIATED ANTIGEN IN IMMUNOPOTENTIATION1982
- IMMUNE-RESPONSE MEDIATED BY LIPOSOME-ASSOCIATED PROTEIN ANTIGENS .1. POTENTIATION OF THE PLAQUE-FORMING CELL RESPONSE1982
- Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucosideBiochemistry, 1981
- Liposomes as immunological adjuvants in eliciting antibodies specific to the synthetic polypeptide poly(LTyr, LGlu)‐poly(DLAla)–poly(LLys) with high frequency of site‐associated idiotypic determinantsEuropean Journal of Immunology, 1981
- LIPOSOMES IN THERAPEUTIC AND PREVENTIVE MEDICINE: THE DEVELOPMENT OF THE DRUG‐CARRIER CONCEPTAnnals of the New York Academy of Sciences, 1978
- Liposomes as immunological adjuvantsNature, 1974
- Use of Chromium Chloride As the Coupling Agent in a Modified Plaque Assay Cells Producing Anti-Protein AntibodyThe Journal of Immunology, 1971