Effect of Chemicals, Heat, and Histopathologic Processing on High-Infectivity Hamster-Adapted Scrapie Virus

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Abstract
High-titered (>1010 LD50 [50% lethal dose]/g) preparations of scrapie virus-infected hamster brain were subjected to inactivation by various chemicals, autoclaving, and histopathologic processing. Sodium hypochlorite, which reduced infectivity by ~4 log LD50/g of brain (99.99%), was somewhat superior to sodium metaperiodate and clearly superior to chlorine dioxide, Lysol® (National Laboratories, Montvale, N.J.), iodine, potassium permanganate, and hydrogen peroxide. Most inactivation occurred within 15–30 min of exposure to a chemical, and little if any additional inactivation occurred after 1 hr. Brains processed for histopathologic examination (formalin fixation followed by dehydration in methanol, clearing in chloroform, and embedding in paraffin) retained ⩾6.810g LD50/g of the infectivity present in unprocessed control tissues (9.6 log LD50/g). One hour in an autoclave at 121 C reduced the titer of scrapie virus by ~7.5 log LD50/g of brain but left 2.5 log LD50/g of residual infectivity. A combination of exposure to chemicals and autoclaving may be necessary to sterilize high-titered scrapie virusinfected tissue.