Hyporesponsiveness to Virus Antigens in Rheumatoid Synovial and Blood Lymphocytes Using the Indirect Leucocyte Migration Inhibition Test

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Abstract
Mononuclear cells (MNC) from rheumatoid synovial tissue and peripheral blood were tested for immune response to various viral antigens, purified protein derivative (PPD) and Mycoplasma pneumoniae by the indirect leukocyte migration inhibition test. MNC from the 11 rheumatoid synovial tissues tested had deficient leukocyte inhibitory factor production against all antigens for which they were tested; this was also the case in the peripheral blood of 7 juvenile rheumatoid arthritis patients (JRA). In the peripheral blood of 8 rheumatoid arthritis (RA) patients there was also generally low reactivity. Significant differences in migration indexes were found with rubella viral antigen and with PPD at 5 .mu.g/ml when zero-hour and overnight incubations of the culture were compared. MNC of peripheral blood of control donors had significant responses to PPD (19/19), mumps virus (7/11), rubella virus (10/19), cytomegalovirus (4/11) and herpes simplex type 1 virus (4/11) antigen after zero-hour culture and no differences was seen after overnight incubation.