Proteins of the 30‐S Subunit of Escherichia coli Ribosomes which Interact Directly with Natural mRNA
- 1 April 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 132 (1) , 139-145
- https://doi.org/10.1111/j.1432-1033.1983.tb07338.x
Abstract
Ultraviolet irradiation (254 nm) of the complexes of MS2 phage RNA (mRNA) and the 30‐S aubunits of Escherichia coli ribosomes prepared at 0°C and 37°C in the presence and absence of intiation factor 3 (IF‐3) causes cross‐linking of mRNA with proteins S3, S4, S5, S7, S9, S18 and IF‐3. Hence, these proteins interact directly with mRNA within the complex 30‐S‐subunit. mRNA.Additin of IF‐3 results in an increase o f the rate of complex formation and decrease of its dissociation rate. The addition of IF‐3 changes the relative amounts of cross‐linked proteins (mainly S3, S4 and S18).Decreasing the temperature from 37°C to 0°C not only decelerates the complex formation rate but also changes the relative amount of cross‐linkes proteins, indicating the influence of the conditins on the structure of the complex.This publication has 45 references indexed in Scilit:
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