Excretion of Specific Urinary Nitrogen Metabolites for Evaluation of Nutritional Treatments

Abstract
Male Holstein calves (16) were assigned to 4 treatments at 3 days old. Treatments were whole milk, an all milk protein commercial replacer, a whey protein milk replacer and a high fat whey protein milk replacer all fed at 10% of body weight. All treatments were isonitrogenous. Chopped wheat straw was fed ad lib. Calves underwent preputial resection for collection of urine. Five day N balance trials were at 1, 5, 9 and 13 wk. Basic nitrogenous components of urine were separated with an amino acid analyzer. Analysis of body weights and straw intakes by balance period showed no differences. Quantitative measurement of excreted basic nitrogenous compounds in urine may improve the evaluation of N metabolism in balance experiments.

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