A Deficient Pyruvate Kinase with an Electrophoretically Slow-Moving Component
- 24 April 2009
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Haematology
- Vol. 19 (1) , 54-60
- https://doi.org/10.1111/j.1600-0609.1977.tb02718.x
Abstract
A deficient erythrocyte pyruvate kinase observed in a patient with congenital nonspherocytic anemia was characterized by very low activity in hemolysates, decreased thermal stability, slightly increased urea denaturation, high affinity for phosphoenolpyruvate poor fructose-1,6-diphosphate activation, normal ATP inhibition, decreased affinity for ADP, normal pH of optimal activity and presence of an abnormal slow-moving component in thin layer polyacrylamide gel electrophoresis. The patient was probably double heterozygous for 2 different deficient mutants of erythrocyte pyruvate kinase.Keywords
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