Quantitation of some Pancreatic Juice Exocrine Proteins in the Presence of Radiocontrast Medium used in Endoscopic Retrograde Pancreatography (ERP)

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Abstract
The endoscopic collection of human pancreatic juice for chemical analysis is facilitated by the use of radiocontrast media. However, the effects of this material on pancreatic juice and the techniques used to analyze its proteins are unknown. No detrimental effects of a commonly used contrast medium on the pancreatic juice itself or on the analytic technique of radioimmunoassay and radial immunodiffusion were demonstrated. Likewise, the Phadebas enzymatic technique for amylase was not affected by this material. Techniques for the collection of pure pancreatic juice using radiocontrast material do not interfere with the analysis of pancreatic juice proteins using the techniques described.