Effects of Thrombin on Factor VIII Activity: Stabilization of Factor VIII by Glycine Ethyl Ester
- 1 January 1967
- journal article
- research article
- Published by Georg Thieme Verlag KG in Thrombosis and Haemostasis
- Vol. 17 (03/04) , 381-387
- https://doi.org/10.1055/s-0038-1654161
Abstract
The action of purified thrombin on a preparation of antihemophilic factor with fibrinogen has been studied. In the absence of active thrombin (thrombin inactivated with DFP) polymerization of fibrin by calcium was accompanied by significant losses of AHF. In the presence of thrombin and calcium, 0.3 M glycine ethyl ester resulted in a significant reduction in the loss of AHF activity. * Present Address: Department of Pediatrics, Cincinnati Childrens Hospital, Cincinnati, Ohio.This publication has 6 references indexed in Scilit:
- Separation of the Antihemophilic Factor (F. VIII) from Fibrinogen With Thrombin and Manganese Chloride.Experimental Biology and Medicine, 1966
- The Interaction of Factors VIII and IXBritish Journal of Haematology, 1964
- The Importance of Activation of Antihemophilic Globulin and Proaccelerin by Traces of Thrombin in the Generation of Intrinsic Prothrombinase ActivityBlood, 1963
- The preparation of human fibrinogen free of plasminogenBiochimica et Biophysica Acta, 1962
- Some Factors that Influence Utilization of Antihemophilic Activity During Clotting.Experimental Biology and Medicine, 1957
- Congenital AfibrinogenemiaBlood, 1954