Genomic Sequence Analysis and Organization of BmKαTx11 and BmKαTx15 from Buthus martensii Karsch: Molecular Evolution of α-toxin genes
- 31 July 2005
- journal article
- research article
- Published by Korean Society for Biochemistry and Molecular Biology - BMB Reports in BMB Reports
- Vol. 38 (4) , 386-390
- https://doi.org/10.5483/bmbrep.2005.38.4.386
Abstract
Based on the reported cDNA sequences of BmKalphaTxs , the genes encoding toxin BmKalphaTx11 and BmKalphaTx15 were amplified by PCR from the Chinese scorpion Buthus martensii Karsch genomic DNA employing synthetic oligonucleotides. Sequences analysis of nucleotide showed that an intron about 500 bp length interrupts signal peptide coding regions of BmKalphaTx11 and BmKalphaTx15. Using cDNA sequence of BmKalphaTx11 as probe, southern hybridization of BmK genome total DNA was performed. The result indicates that BmKalphaTx11 is multicopy genes or belongs to multiple gene family with high homology genes. The similarity of BmKalpha-toxin gene sequences and southern hybridization revealed the evolution trace of BmKalpha-toxins: BmKalpha-toxin genes evolve from a common progenitor, and the genes diversity is associated with a process of locus duplication and gene divergence.Keywords
This publication has 15 references indexed in Scilit:
- Expression of an antitumor–analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coliProtein Expression and Purification, 2003
- An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi KarschToxicon, 2002
- Electrophysiological characterization of BmK M1, an α‐like toxin from Buthus martensi Karsch venomFEBS Letters, 2001
- Crystal structures of two α-like scorpion toxins: non-proline cis peptide bonds and implications for new binding site selectivity on the sodium channel 1 1Edited by R. HuberJournal of Molecular Biology, 1999
- Cloning and characterization of the genomic region encoding toxin IV-5 from the scorpion Tityus serrulatus Lutz and MelloToxicon, 1996
- Promoter structure and intron-exon organization of a scorpion .alpha.-toxin geneBiochemistry, 1995
- Crystal Structure of Toxin II from the Scorpion Androctonus australis Hector Refined at 1·3 Å ResolutionJournal of Molecular Biology, 1994
- Nuclear pre-mRNA introns: analysis and comparison of intron sequences fromTetrahymena thermophilaand other eukaryotesNucleic Acids Research, 1990
- Primary structure of toxin IV of Leiurus quinquestriatus quinquestriatusFEBS Letters, 1985
- Two types of scorpion toxin receptor sites, one related to the activation, the other to the inactivation of the action potential sodium channelToxicon, 1982