Comparative calorimetric studies on the dynamic conformation of plant 5S rRNA: II structural interpretation of the thermal unfolding patterns for lupin seeds and wheat germ

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Abstract
Thermal unfolding of 5S rRNA from wheat germ (WG) and lupin seeds (LS) was studied in solution. Experimental curves of differential scanning calorlmetry (DSC) were resolved into particular components according to the thenmodynamic model of two-state transitions. The DSC temperature profiles for WG and LS differ significantly in spite of very high similarities in the sequence of both molecules. Those results are Interpreted according to a model of the secondary and tertiary molecular structure of 5S rRNA. A comparison of the ‘nearest neighbour’ model of Interaction with the experimental thermodynamlc results enables a complete interpretation of the process of the melting of its structures. In light of our observations, the crucial differences between both DSC melting profiles are mainly an outcome of different thermodynamic properties of the first helical fragment ‘A’ made up of 9 complementary base pairs. It contains 6 differences in the nucleotide sequence of both types of molecules, which still retain 9-meric double helixes. The temperature stability of his helix In WG Is much lower than of the LS one. Moreover, the results supply evidence for a strong specific tertiary interaction between the two hairpin loops ‘C’ and ‘e’ in both 5S rRNA molecules, modulated by small differences in the thermodynamic properties of both 5S rRNA.

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