Ion-Exchange High Performance Liquid Chromatographic Determination of Selenomethionine by Reaction with Cyanogen Bromide
- 1 November 1992
- journal article
- separations
- Published by Taylor & Francis in Analytical Letters
- Vol. 25 (11) , 2165-2174
- https://doi.org/10.1080/00032719208020083
Abstract
A sensitive and simple ion-exchange HPLC procedure is described for the separation and analyses of selenomethionine from coeluting naturally occurring amino acids. Cyanogen bromide selectively reacts with selenomethionine in the absence of stannous chloride to form an unknown reaction product, formation of which is quantitative. A calibration curve is obtained over a linear dynamic range of 30 nmoles to 500 nmoles of selenomethionine.Keywords
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