THYMIDINE-SUICIDE OF HUMAN GRANULOCYTIC PROGENITOR CELLS (CFU-C)

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 28  (6) , 319-323
Abstract
The quantitative and qualitative changes of human CFU-C after perturbation gave a comprehensive view of their reaction pattern. The proliferative state of CFU-C could be assessed using the 3H-thymidine (3H-TdR) technique. This technique is well established in experimental animals. To utilize it for the study of human CFU-C, the kinetics and the variables affecting the suicide of human CFU-C were analyzed in detail. It appeared that under optimal conditions considerably higher 3H-TdR concentrations (.gtoreq. 100/.mu.Ci per ml) have to be utilized than have been reported to be satisfactory in the murine system. Colony growth inhibition was S-phase-specific. If reutilization of the label occurs in vitro, it does not detectably affect colony growth. Minimum exposure of CFU-C to 3H-TdR was 20 min. Using these optimal suicide conditions, human marrow CFU-C can be a highly proliferating cell population.