Quantitation of Immunoglobulin Classes and Subclasses in Anti‐Rh (D) Antibodies1

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Abstract
The isotype profiles of anti‐Rh (D) antibodies were measured from 16 serum samples with a solid‐phase radioimmunoassay that employed monoclonal anti‐isotype antibodies. These antibodies had been standardized in another solid‐phase system with the aid of monoisotypic antibodies (e.g., anti‐tetanus toxoid of isotype IgG4), and this permitted calculations of the relative concentrations of different isotypes in each anti‐D population. Of the heavy‐chain isotypes, only IgG1 and IgG3 were found in anti‐Rh (D) antibodies. The share of IgG1 varied from 100% (2 sera) to 0% (1 serum) of detected units, and the mean proportion was 73.8%. The remainder was IgG3 (mean share 26.2%). The proportions of kappa and lambda immunoglobulins varied greatly in individual sera, in three sera only kappa Ig and in one serum only lambda Ig could be detected.