Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa
Open Access
- 1 March 1973
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 51 (3) , 591-593
- https://doi.org/10.1104/pp.51.3.591
Abstract
The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO3− in the NADH-nitrate reductase assay are 10 μm, 2.6 μm, and 0.23 mm, respectively. Heat treatment exerts varying effects on the enzymatic activities associated with the nitrate reductase complex.Keywords
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