A Physical Map of the Filamentous Bacteriophage Cf Genome

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Abstract
A total of 23 restriction endonucleases were used to cleave the genome of bacteriophage Cf. BamHI, EcoRI, HaeII, KpnI, PstI, SmaI and SstII each cut Cf DNA at 1 site; HindIII, PvuI, PvuII and XhoI cleaved at 2 sites; BglII cleaved at 3 sites; HincII cleaves at 4 sites; and HinfI, BstNI, AluI, HpaII and HaeIII cleaved at 14, 19, 20, 24 and 26 sites, respectively. HpaI and XbaI do not cleave the Cf genome. HhaI and TaqI cleaved at > 20 and > 8 sites, respectively. Based on the sizes of the fragments produced by digestion with these enzymes, Cf DNA was estimated to have 7.62 .+-. 0.1 kilobase pairs. Using the EcoRI cleavage site as a reference point, 9 restriction enzymes, BamHI, BglII, EcoRI, HindIII, KpnI, PstI, PvuII, SstII and XhoI were selected and a detailed physical map of the Cf genome was constructed.