Isolation of Glycoalkaloids with the Chromatotron and Their Determination by High-Performance Liquid Chromatography

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Abstract
An extract of Solanum laciniatum leaves was fractionated with the Chromatotron. The glycoalkaloid fraction thus obtained was analyzed by reversed-phase HPLC [high-performance liquid chromatography]. The load capacity of the Chromatotron with a 2-mm layer thickness is 600 mg (as determined with cholesterol), and the recovery of solasonine from the plant extract (as determined by HPLC) is 93-96%. The HPLC method permits the detection of as little as 1.5 .mu.g solasonine or 3.5 .mu.g solamargine with a linear detector response up to 375 .mu.g for the former and 250 .mu.g for the latter.