Na+/H+ antiporter is the primary proton transport system used by osteoclasts during bone resorption
- 4 February 1990
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 142 (2) , 420-424
- https://doi.org/10.1002/jcp.1041420227
Abstract
We have examined the effects of inhibitors of proton transport systems on osteoclastic bone resorption using an in vitro bone slice assay, where osteoclasts (OCs) are free from the influence of other bone cells. Amiloride (AM) and dimethyl‐amiloride (DMA), inhibitors of the Na+ /H+ antiporter, were potent inhibitors of bone resorption (IC50 ∼ 9 and 0.7 μM for AM and DMA, respectively). Omeprazole (OM), a potent inhibitor of parietal cell K+ /H+ ‐ATPase, was a poor inhibitor of OC bone resorption (IC50 ∼ 100 μM). These results strongly suggest that the Na+ /H+ antiporter is the primary proton system used by OCs during bone resorption.Keywords
This publication has 28 references indexed in Scilit:
- Na+/H+ exchange and growth factor-induced cytosolic pH changes. Role in cellular proliferationPublished by Elsevier ,2003
- Basolateral membrane Na+/H+ antiport, Na+/base cotransport, and Na+-independent Cl-/base exchange in the rabbit S3 proximal tubule.Journal of Clinical Investigation, 1989
- Cytoplasmic pH regulation and chloride/bicarbonate exchange in avian osteoclasts.Journal of Clinical Investigation, 1989
- Cellular Biology and Biochemical Mechanism of Bone ResorptionClinical Orthopaedics and Related Research, 1988
- Structure—activity relationships of omeprazole analogues and their mechanism of actionTrends in Pharmacological Sciences, 1987
- Parathyroid hormone stimulation of the Na+/K+ pump in rat clonal osteosarcoma cellsJournal of Endocrinology, 1986
- Isolated osteoclasts resorb the organic and inorganic components of bone.The Journal of cell biology, 1986
- Kinetic Properties of the Plasma Membrane Na+ -H+ ExchangerAnnual Review of Physiology, 1985
- Current Studies on the Location and Function of Carbonic Anhydrase in OsteoclastsaAnnals of the New York Academy of Sciences, 1984
- Parathyroid hormone stimulates bone resorption via a Na–Ca exchange mechanismNature, 1980