Modulation of Herpes Simplex Virus Replication in Adenovirus Transformed Cells

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Abstract
The ability of herpes simplex virus [HSV] type 1 to replicate in cells transformed by adenovirus type 5 is strongly dependent on the origin of the cells. Studies show that adenovirus transformed rat cells lose their permissiveness while cells of hamster or human origin retain their ability to replicate HSV, although at a reduced level when compared to the untransformed parent cells. One line of adenovirus transformed rat [embryo fibroblast] cells, 107, demonstrates thermosensitive events, allowing HSV to replicate at 34.degree. C but not at 37.degree. C. Analysis of the biochemical events taking place at 37.degree. C showed that virus-specific DNA synthesis was greatly reduced but that all of the late virus structural proteins could be observed after SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis. Shut-off of host macromolecular synthesis appeared to be less efficient after HSV infection of 107 cells than after infection of more permissive cells such as the non-transformed REF [rat embryo fibroblast] line. Collectively the data show that interactions between HSV and the host cell are perturbed when the cell is tranformed by type 5 adenovirus. The degree of perturbation ranges from a slight reduction in number of progeny to a completely abortive infection.