A bio-engineered rubella E1 antigen

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Abstract
The major rubella envelope protein, E1, and a segment of it, comprising amino acids 207–353, have been separately expressed as fusion proteins with the IgG binding region ofStaphylococcus aureus protein A inEscherichia coli. The proteins carry E1-specific antigenicity recognized by monoclonal antibodies raised against whole virus confirming that correct glycosylation is not required for antigenicity. The use of these bioengineered antigens in immunoassays for diagnosis of rubella infection and for immunization in experimental animals is described.