Simple and Fast Determination of Some Phenethylamines in Illicit Tablets by Base-Deactivated Reversed Phase HPLC

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Abstract
A high performance liquid chromarographic (HPLC) method for the simultaneous identification and quantitation of amphetamine, methamphetamine, 3, 4-methylenedioxyamphetamine (MDA), N-methyl-3,4-methylenedioxyamphetamine (MDMA), and N-ethyl-3,4-methylenedioxyamphetamine (MDE) in presence of caffeine and ephedrine in illicit tablets is described. A simple and rapid sample preparation procedure was applied in order to allow a high number of samples to be processed per day. The chromatographic separation was performed on a commercially available base-deactivated octadecyl silica column with a gradient system using acetonitrile and 20 mM monobasic potassium phosphate buffer. The flow rate was 1.5 ml/min and peak detection was performed at 220 and 280 nm. Peak identity and homogeneity were determined by mapping of peaks in the 195–370 nm range. Quantitative analysis was performed using external standard method in the concentration range studied. Linearity was evaluated in the range 10–500 μg/ml for the 5 substances in exam and for ephedrine, and in the range 3–100 μg/ml for caffeine. Correlation coefficients ranged from 0.9985 to 0.9999. Good accuracy and between-day precision were achieved.

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