HORSERADISH-PEROXIDASE - KINETIC-STUDIES AND OPTIMIZATION OF THE ACTIVITY DETERMINATION WITH THE SUBSTRATES H2O2 AND O-PHENYLENEDIAMINE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 20  (4) , 221-225
Abstract
To optimize the determination of the activity of peroxidase, an enzyme often used as a marker and indicator system in enzyme immunoassays, the kinetics of the catalytic reaction and its dependence on the H2O2 and o-phenylenediamine concentration at various pH values were investigated. In addition, the influence of buffer systems, the reaction temperature, and the reaction time were determined. Based on the results, a test procedure is proposed for the measurement of peroxidase down to concentrations of 2 ng/l.

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