The biochemical effects of CPDA‐2‐drawn red blood cells of delayed refrigeration prior to component preparation

Abstract
The effects of an 8-h hold at 22.degree. C prior to component preparation were evaluated in a split-bag study using 9 U of blood preserved in citrate-phosphate-dextrose-adenine (CPDA-2). Each unit was divided in half, platelet-rich plasma removed at 0 or 8 h, respectively, and the half units of red blood cells stored at 4.degree. C for 42 days. The only red blood cell metabolic differences seen in the bags held 8 h (compared to those not held) were a 21% rise in ATP, which was not significant after 14 days of storage, and a 33% loss of 2,3-diphosphoglycerate which resulted in a loss curve similar to that seen with acid-citrate-dextrose blood. The logistic advantages seem to warrant an 8-h holding period for red blood cells drawn in CPDA-2.