RNA polymerase and an activator form discrete subcomplexes in a transcription initiation complex
Open Access
- 3 August 2006
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 25 (16) , 3784-3790
- https://doi.org/10.1038/sj.emboj.7601261
Abstract
Using high‐resolution atomic force microscopy (AFM) we show that in a ternary complex of an activator protein, FIS, and RNA polymerase containing the σ70 specificity factor at the Escherichia coli tyrT promoter the polymerase and the activator form discrete, but connected, subcomplexes in close proximity. This is the first time that a ternary complex between an activator, a σ70 polymerase holoenzyme and promoter DNA has been visualised. Individually FIS and RNA polymerase wrap ∼80 and 150 bp of promoter DNA, respectively. We suggest that the architecture of the ternary complex provides a general paradigm for the facilitation of direct, but weak, interactions between polymerase and an activator.Keywords
This publication has 36 references indexed in Scilit:
- DNA looping-mediated repression by histone-like protein H-NS: specific requirement of Eσ70 as a cofactor for loopingGenes & Development, 2005
- The regulation of bacterial transcription initiationNature Reviews Microbiology, 2004
- Structural Basis of Transcription Activation: The CAP-αCTD-DNA ComplexScience, 2002
- FIS Modulates the Kinetics of Successive Interactions of RNA Polymerase with the Core and Upstream Regions of the tyrT PromoterJournal of Molecular Biology, 2002
- Contributions of UP Elements and the Transcription Factor FIS to Expression from the Seven rrn P1 Promoters in Escherichia coliJournal of Bacteriology, 2001
- Transcriptional activation via DNA-looping: visualization of intermediates in the activation pathway of E. coli RNA polymerase·σ54 holoenzyme by scanning force microscopyJournal of Molecular Biology, 1997
- Escherichia coli tyrT gene transcription is sensitive to DNA supercoiling in its native chromosomal context: effect of DNA topoisomerase IV overexpression on tyrT promoter functionMolecular Microbiology, 1994
- RNA polymerase interactions with the upstream region of the E. coli tyrT promoterCell, 1983
- Requirement for an upstream element for optimal transcription of a bacterial tRNA geneNature, 1983
- The Influence of Ribonucleoside Triphosphates, and Other Factors, on the Formation of Very‐Salt‐Stable RNA‐Polymerase · su+III‐tRNA(tRNATyr)‐Promoter ComplexesEuropean Journal of Biochemistry, 1979