DETECTION, ASSAY, AND PRELIMINARY PURIFICATION OF THE PIGMENT CONTROLLING PHOTORESPONSIVE DEVELOPMENT OF PLANTS

Abstract
Instrumentation and procedures are described for the detection of the photoreversible pigment in intact tissue with a recording single-beam spectrophotometer and the assay based on the measurement of the optical density difference between 2 fixed wavelengths using a double-beam, bichromatic spectrophotometer. A system for separation of the pigment using usual methods of protein chemistry is described.

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