MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI

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Abstract
A series of experiments are described in which the density gradient centrifugation technique is used to investigate the mechanism of DNA replication in the chromosomes of the unicellular green alga, Chlamydomonas reinhardi. Only mitotic replication mechanisms are reported in the present paper; the investigations of meiotic replications will appear later. Methods of isolation and properties of DNA are described and the results of transfer experiments from a nitrogen-15 (N ) medium to a nitrogen-14 (N14) medium are presented. The transfer experiment on mitosis of C. reinhardi gives a pattern of DNA replication analogous to that of E. coli - a pattern in which each continuous half of the DNA subunit is conserved in two daughter molecules of the succeeding generation.