Erythropoietic Activity of Tissue Homogenates.

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Abstract
Homogenates of rat liver, kidney, brain, spleen and muscle prepared in modified Tyrodes solution and injected into the dehydrated rat are capable of stimulating erythropoiesis as measured by Fe59 incorporation into red cells and reticulocyte counts. Control animals average a 5.65% 24 hour Fe59 incorporation with a 0.4% reticulocyte count. Animals receiving homogenates average a 3-4 fold increase in iron incorporation and a 4-8 fold increase in reticulocyte levels. Similar activity is noted in homogenates prepared from monkey kidney and liver. The effect is not achieved by the administration of bovine albumin, gum acacia, or methylcellulose. The homogenate response does not then appear to be non-specific. Homogenate activity is retained following acidification and boiling. In this respect the activity is similar to the plasma factor, erythro-poietin.