VARIATION OF BOUND COAGULASE OF STAPHYLOCOCCUS AUREUS

Abstract
Regardless of original bound coagulase (clumping factor) titer, each of 16 coagulase-positive strains of S. aureus either partially or completely lost bound coagulase activity after storage of agar cultures and frozen cell suspensions under a variety of conditions including refrigeration and deep freeze at -26 and -55[degree]C. The rate of reduction in bound coagulase titer varied with individual strains. The original free coagulase activity remained unchanged. Decrease of pigmentation was not always associated with decrease in bound coagulase titer. At the end of the storage period, the bound coagulase titers of organisms grown from lyophilized cultures agreed with those of the parent strains. Transfer through 24 subcultures failed to increase the bound coagulase activity of 3 of 9 storage induced, bound coagulase deficient variants, whereas the activity of the other 6 was either partially or completely restored by subculture.

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