ANIMAL-MODEL FOR HUMAN OSTEOSARCOMA

  • 1 January 1977
    • journal article
    • research article
    • Vol. 81  (2) , 168-175
Abstract
Osteosarcomas formed in antilymphocyte serum (ALS) treated hamsters when 2 .times. 106 TE-85 human osteosarcoma cells (maintained in tissue culture) infected with M-MSV [Moloney murine sarcoma virus] (RD-114) virus were injected adjacent to the femur or scapula. Undifferentiated sarcomas formed when 1 .times. 106 cells were injected s.c. Tumors were palpable 10-14 days after the cells were injected and grew progressively until the animals died (mean survival time was 30 days). All animals had pulmonary metastases. Neither the subcutaneous sarcomas nor the metastases contained bone or osteoid. Osteosarcomas adjacent to the femur and scapula contained collagen, osteoid and calcified bone when observed by light microscopy and EM. These results indicate that the TE-85-M-MSV cell-ALS hamster system is an animal model for the study of osteosarcomas of human cell origin.