Assay of adenosine 3′-phosphate 5′-sulphatophosphate in hepatic tissues
- 1 July 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 181 (1) , 107-110
- https://doi.org/10.1042/bj1810107
Abstract
A fluorimetric assay, based on the ability of boiled hepatic extracts to support the sulphO-conjugation of harmol, was used to demonstrate and quantify PAdoPS (adenosine 3′-phosphate 5′-sulphatophosphate) present in liver. A stoicheiometric relationship was established between the sulphate conjugate formed and the ‘active sulphate’ utilized. Guinea-pig, rat, mouse and rabbit livers contain 3.3, 2.9, 0.8 and 0.5 mumol of PAdoPS/100 G wet wt. respectively.This publication has 23 references indexed in Scilit:
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