Effect of insulin of glucose and glycogen metabolism and leucine incorporation into protein in cultured mouse astrocytes
- 1 January 1992
- Vol. 6 (4) , 264-268
- https://doi.org/10.1002/glia.440060404
Abstract
Insulin, following binding to its receptor, produces a dose- and time-dependent stimulation of entry of 2-deoxy-D-[U-14C] glucose and glycogen synthesis from D-[U-14C] glucose in cultured mouse astrocytes following differentiation. Maximal stimulation of both glucose entry (217% above basal) and of glycogen synthesis (209% above basal) was observed at an insulin concentration of 1.7 × 10−8 M. Insulin also stimulates the incorporation of leucine into astrocytic proteins with maximal stimulation (156% above basal) at an insulin concentration of 1.7 × 10−7 M, but no effect on leucine uptake was observed at an insulin concentration of 1.7 × 10−6 M. These results, together with a previous demonstration that insulin and certain insulin analogues stimulate pyrimidine nucleoside incorporation into nucleic acid, indicate that insulin has diverse actions on biomacromolecular metabolism in cultured mouse astrocytes.Keywords
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