Effect of Aldosterone on the Human Erythrocyte Sodium-Potassium Pump in vitro
- 1 February 1983
- journal article
- research article
- Published by Portland Press Ltd. in Clinical Science
- Vol. 64 (2) , 183-186
- https://doi.org/10.1042/cs0640183
Abstract
The effects of aldosterone in vitro on the Na+,K+-dependent ATPase activity of isolated human erythrocyte membranes and on 86Rb uptake and [3H]ouabain binding of intact erythrocytes were studied. ATPase activity was nearly doubled (0.061 .+-. 0.006 to 0.110 .+-. 0.01 .mu.mol of Pi h-1 mg-1 of protein) by the addition of a physiological concentration of aldosterone (2.7 .times. 10-10 mol/l). Higher concentrations had no greater effect. Aldosterone had no significant effect on 86Rb uptake or [3H]ouabain binding. Erythrocytes contain aldosterone at concentrations similar to that in plasma. The effect of aldosterone on ATPase is probably maximal.This publication has 10 references indexed in Scilit:
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