PCR-based microsatellite polymorphisms in the detection of loss of heterozygosity in fresh and archival tumour tissue
Open Access
- 1 August 1993
- journal article
- Published by Springer Nature in British Journal of Cancer
- Vol. 68 (2) , 308-313
- https://doi.org/10.1038/bjc.1993.333
Abstract
PCR-based microsatellite polymorphisms have proved their power in genetic linkage analysis and other identification methods, due to their high information content and even distribution over the chromosomes. In the present study we applied microsatellite polymorphisms to detect loss of heterozygosity in fresh (snap-frozen) and in archival ovarian tumour tissue. Clear allele losses were found in fresh and paraffin embedded tumour samples. Conventional Southern analysis of flanking markers on the same tumour DNA samples confirmed the observed losses detected by microsatellite polymorphisms. Titration experiments suggest that loss of heterozygosity remains detectable in tumour samples despite 60% contamination with normal DNA. This technique provides a fast and reproducible alternative to conventional Southern blotting in the detection of loss of heterozygosity, with the crucial additional advantages of minimal sample requirements, making archival material available for genetic investigation.Keywords
This publication has 32 references indexed in Scilit:
- Assignment of a Locus for Familial Melanoma, MLM, to Chromosome 9p13-p22Science, 1992
- Allele loss on chromosome 17q in sporadic ovarian cancerThe Lancet, 1991
- PCR amplification from paraffin-embedded tissues: recommendations on fixatives for long-term storage and prospective studies.Genome Research, 1991
- Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction.Journal of Histochemistry & Cytochemistry, 1991
- The murine situs inversus viscerum (iv) gene responsible for visceral asymmetry is linked tightly to the Igh-C cluster on chromosome 12Genomics, 1990
- At least four different chromosomal regions are involved in loss of heterozygosity in human breast carcinomaGenomics, 1989
- Genetic Alterations during Colorectal-Tumor DevelopmentNew England Journal of Medicine, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- A study of the evolution of repeated DNA sequences in primates and the existence of a new class of repetitive sequences in primatesJournal of Molecular Biology, 1979