DNA sequence of the int-xis-P1region of the bacteriophage lambda; overlap of the int and xis genes
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (8) , 1765-1782
- https://doi.org/10.1093/nar/8.8.1765
Abstract
The DNA sequence of the int and xis genes of lambda bacteriophage, as well as that of the PI promoter and a region of unknown function beyond this, was determined by the chain termination procedure. The Pribnow box sequence of the PI promoter lies just within the xis gene, and both possible sites of mRNA initiation from PI are within the xis gene. The end of the xis gene in its turn overlaps the start of the int gene by 23 base pairs, in a different reading frame. This overlap may play a role in ensuring efficient excision of the prophage in response to natural induction signals.Keywords
This publication has 31 references indexed in Scilit:
- Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coliJournal of Molecular Biology, 1975
- Terminal-Sequence Analysis of Bacterial Ribosomal RNA. Correlation between the 3'-Terminal-Polypyrimidine Sequence of 16-S RNA and Translational Specificity of the RibosomeEuropean Journal of Biochemistry, 1975
- Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonucleaseJournal of Molecular Biology, 1975
- Prophage lambda at unusual chromosomal locationsJournal of Molecular Biology, 1973
- New mutants of bacteriophage λ with a specific defect in excision from the host chromosomeJournal of Molecular Biology, 1970
- Evidence for a prophage excision gene in λJournal of Molecular Biology, 1970
- Integrative and excisive recombination by bacteriophage λ: Evidence for an excision-specific recombination proteinJournal of Molecular Biology, 1970
- SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORMProceedings of the National Academy of Sciences, 1969
- Mutants of bacteriophage lambda unable to integrate into the host chromosome.Proceedings of the National Academy of Sciences, 1967
- Integration-negative (int) mutants of phage λVirology, 1967