Serotonin Binding to Nerve-Ending Particles of the Rat Brain and Its Inhibition by Lysergic Acid Diethylamide
- 29 May 1964
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 144 (3622) , 1135-1137
- https://doi.org/10.1126/science.144.3622.1135
Abstract
The binding of serotonin to nerve-ending particles and other preparations from rat brain has been examined. By investigating the amount bound as a function of serotonin concentration from 10 -7 M to 10 -2 M , it was possible to identify three major components having K assoc (association constant) values of 2 × 10 6 , 5 × 10 4 , and 5 × 10 2 . The component having the highest binding constant was not present in liver and appeared to be confined to the cortex and midbrain regions. This component is inhibited by d -lysergic acid diethylamide at low concentrations. Solubilization of this binding component has been achieved.Keywords
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