Comparative Approach to the Evaluation of Hemoglobin A2 by Two Different Methods: High-Performance Liquid Chromatography and DE-52 Microchromatography
- 1 January 1990
- journal article
- research article
- Published by S. Karger AG in Acta Haematologica
- Vol. 83 (4) , 179-182
- https://doi.org/10.1159/000205209
Abstract
Hb A2 was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of β -thalassemia trait and 228 were normal controls. Hb A2 was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies.Keywords
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