MULTIPLE EFFECTS OF FLUORESCENT LIGHT ON REPAIR OF ULTRAVIOLET‐INDUCED DNA LESIONS IN CULTURED GOLDFISH CELLS
- 2 January 1995
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 61 (1) , 79-83
- https://doi.org/10.1111/j.1751-1097.1995.tb09246.x
Abstract
Abstract— It is known that fluorescent light illumination prior to UV irradiation (FL preillumination) of cultured fish cells increases photorepair (PR) ability. In the present study, it was found that FL preillumination also enhanced UV resistance of logarithmically growing cells in the dark. This enhancement of UV resistance differs from induction of PR because it was not suppressed by cycloheximide (CH) and it occurred immediately after FL preillumination. The effects of FL preillumination on repair of UV‐induced DNA lesions in the dark were examined by an endonuclease‐sensitive site assay to measure the repair of cyclobutyl pyrimidine dimers, and by enzyme‐linked immunosorbent assay to quantitate the repair of (6‐4) photoproducts. It was found that excision repair ability for (6‐4) photoproducts in the genome overall was increased by FL preillumination. Moreover, a decrease in (6‐4) photoproducts by FL illumination immediately after UV irradiation of the cells was found, the decrement being enhanced by FL preillumination with or without CH.This publication has 17 references indexed in Scilit:
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