A method for isolation of oligo(uridylic acid)-containing messenger ribonucleic acid from Hela cells
- 15 September 1981
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 20 (19) , 5359-5364
- https://doi.org/10.1021/bi00522a001
Abstract
When cytoplasmic polyadenylated RNA [poly (A+) RNA] from [human cervical cancer] HeLa cells was treated with RNase H and oligo(dT) to remove its 3''-poly(A) tail, an increased binding to poly(A)-agarose was observed. The bound material, which comprised 4-6% of the initial RNA, contained 65-80% of the oligo(U) sequences generated by RNase T1 digestion. Oligo(U) isolated from the bound fraction was 83% U and had a U/G ratio of 33. In contrast, oligo(U) from the unbound material was 77% U and had a U/G ratio of 13, suggesting that it is shorter and less U rich than the oligo(U) in the bound fraction. On sucrose gradients, oligo(U+) RNA consistently sedimented with a larger s value than oligo(U-) RNA. The oligo(U) content of oligo(U+) RNA suggests one oligo(U) tract of 33 nucleotides per RNA molecule of 2000-3000 residues.This publication has 10 references indexed in Scilit:
- 5'-Terminal cap structures of oligo(uridylic acid)-containing messenger ribonucleic acid from Hela cells: comparison with other ribonucleic acid subpopulationsBiochemistry, 1981
- Unusual nucleotide sequences at the 5' end of actin genes in Dictyostelium discoideum.Proceedings of the National Academy of Sciences, 1979
- The evolution and sequence comparison of two recently diverged mouse chromosomal β-globin genesCell, 1979
- Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.Proceedings of the National Academy of Sciences, 1978
- The absolute frequency of labeled N-6-methyladenosine in HeLa cell messenger RNA decreases with label timeJournal of Molecular Biology, 1978
- Isolation and characterization of the oligo(dA-dT) clusters and their flanking DNA segments in the rabbit genomeJournal of Molecular Biology, 1977
- Heterogeneous nuclear RNA secondary structure: oligo (U) sequences base-paired with poly (A) and their possible role as binding sites for heterogeneous nuclear RNA-specific proteins.Proceedings of the National Academy of Sciences, 1977
- Poly(A)-binding RNAs from nuclei and polysomes of BHK-21 cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Poly (A)-rich ribonucleoprotein complexes from HeLa cell messenger RNA.Journal of Biological Chemistry, 1976
- On the nature of 5′ termini in nuclear pre-mRNA of Ehrlich carcinoma cellsCell, 1976