LACTIC-DEHYDROGENASE IN NORMAL AND LEUKEMIA LYMPHOCYTE SUB-POPULATIONS - EVIDENCE FOR THE PRESENCE OF ABNORMAL T-CELLS AND B-CELLS IN CHRONIC LYMPHOCYTIC-LEUKEMIA

  • 1 January 1981
    • journal article
    • research article
    • Vol. 57  (2) , 324-327
Abstract
Lactic dehydrogenase (LDH) was quantitated and the isozyme pattern studied in lymphocyte subpopulations from normal people and patients with chronic lymphocytic leukemia (CLL). Normal T lymphocytes differed from normal B lymphocytes in having greater total LDH activity (597.2 vs. 252.1). Total LDH activity in CLL T cells (347.1) was lower than in normal T cells but not significantly different than normal B cells. Total LDH activity in CLL B cells (124.6) was lower than normal T cells but not significantly different than normal B cells. Total LDH activity in CLL B cells (124.6) was lower than normal B cells and normal T cells. The isozyme pattern of normal T lymphocytes showed a higher activity in the LDH-1 band but showed a lower activity in the LDH-5 band compared to normal B cells. Chronic lymphocytic leukemia T cells could be distinguished from CLL B cells by a high LDH-5 band and from normal T cells by a high LDH-5 band and from normal T cells by a high LDH-5 band and a low LDH-1 band. CLL B cells could be distinguished from normal B cells by a low LDH-5 band. The LDH isozyme pattern distinguishes normal T lymphocytes from normal B lymphocytes and normal T and B lymphocytes from CLL T and B lymphocytes.