Short-Term Storage of Six Penicillins and Cephalothin in Microdilution Trays for Antimicrobial Susceptibility Tests
Open Access
- 1 July 1976
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 10 (1) , 83-88
- https://doi.org/10.1128/aac.10.1.83
Abstract
To perform microdilution antimicrobial susceptibility tests efficiently, broth dilutions of the antimicrobial drugs should be prepared in large batches and stored frozen until needed. Studies were carried out to document the stability of ampicillin, benzylpenicillin, carbenicillin, methicillin, oxacillin, nafcillin, and cephalothin in microdilution trays during storage at −20 and −60 C. No significant deterioration was noted after 21 days of storage, as detected by bioassay and by replicate minimal inhibitory concentration determinations with control organisms. No significant differences were noted moreover between trays stored for three weeks at −20 C and those stored at −60 C. The microdilution technique was found to be a highly reproducible method for quantitative determination of antimicrobial susceptibility.Keywords
This publication has 5 references indexed in Scilit:
- Microdilution antibiotic susceptibility test: examination of certain variables.1973
- Detailed methodology and implementation of a semiautomated serial dilution microtechnique for antimicrobial susceptibility testing.1970
- A Microdilution Method for Antibiotic Susceptibility Testing: An EvaluationAmerican Journal of Clinical Pathology, 1970
- Semiautomatic microtechnique for serial dilution-antibiotic sensitivity testing in the clinical laboratory.1968
- Antibiotic susceptibility testing by a standardized single disk method.1966