Luminography – a new, highly sensitive visualization method for electrophoresis
- 1 January 1991
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 12 (5) , 367-372
- https://doi.org/10.1002/elps.1150120508
Abstract
A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light‐emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X‐ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.Keywords
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