Newly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis
- 1 January 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (1) , 252-254
- https://doi.org/10.1128/jcm.01489-06
Abstract
New rpoB gene primers for detecting Rif r in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif r isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.Keywords
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