Development of a Surface Adhesion Immunofluorescent Technique for the rapid detection ofSalmonellaspp. from meat and poultry
Open Access
- 1 April 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 86 (4) , 583-590
- https://doi.org/10.1046/j.1365-2672.1999.00698.x
Abstract
A rapid method based on bacterial adhesion was developed for the detection of Salmonella in an enriched meat system. Minced beef samples inoculated with Salm. enteritidis (10 cfu g−1) were incubated overnight (18 h) at 37 °C in buffered peptone water. Salmonella enteritidis cells were isolated from the enriched meat sample by surface adhesion onto a polycarbonate membrane attached to a glass slide. The organisms attached to this polycarbonate membrane were subsequently visualized using immunofluorescent microscopy. The technique had a detection level of log10 3·5 Salmonella ml−1. The surface adhesion immunofluorescent technique correlated well with Salmonella plate counts (r2 = 0·99). Application of the rapid method to retail beef and poultry samples (n = 100) confirmed the correlation between this technique and traditional microbiological procedures. Thirty‐one retail samples were reported positive for Salmonella species. No false positives or negatives were recorded for the rapid method.Keywords
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