5' flanking sequence and genomic structure of Egr-1, a murine mitogen inducible zinc finger encoding gene
Open Access
- 26 September 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (18) , 8835-8846
- https://doi.org/10.1093/nar/16.18.8835
Abstract
Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and consists of 2 exons and one 700 bp intron. S1 nuclease protection and primer extension analysis were used to define the transcription initiation site. “TATA” and “CCAAT” sequences were located at nucleotides −26 and −337 respectively. In addition, there exist five elements whose sequence is nearly identical to the inner core 10 nucleotide region (CCATATTAGG) of the c-fos serum response element, four Spl consensus sequences, two AP1 target sequence analogs, and two potential cAMP response elements. These results will ultimately lead to a detailed definition of the intracellular events regulating Egr-1 expression.Keywords
This publication has 24 references indexed in Scilit:
- Upstream sequences required for transcription of the TFIIIA genein XenopusoocytesNucleic Acids Research, 1988
- The sex-determining region of the human Y chromosome encodes a finger proteinCell, 1987
- Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domainCell, 1987
- A Nerve Growth Factor-Induced Gene Encodes a Possible Transcriptional Regulatory FactorScience, 1987
- Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin geneNature, 1987
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factorCell, 1987
- The fos OncogenePublished by Elsevier ,1987
- Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factorsCell, 1986
- A catalogue of splice junction sequencesNucleic Acids Research, 1982