Assessment of Renal Functional Phenotype in Mice Lacking gp91 PHOX Subunit of NAD(P)H Oxidase
- 1 February 2004
- journal article
- research article
- Published by Wolters Kluwer Health in Hypertension
- Vol. 43 (2) , 335-340
- https://doi.org/10.1161/01.hyp.0000111137.15873.4a
Abstract
To determine the role of endogenous superoxide (O 2 − ) in the kidney, we assessed renal hemodynamics and excretory function in gp91 PHOX (a NAD(P)H oxidase subunit) gene knockout (KO) mice and compared these findings with those of wild-type (WT) strain C57BL/6 mice. Renal blood flow (RBF) and glomerular filtration rate (GFR) were determined by PAH and inulin clearances respectively in anesthetized mice (n=8 in each group). There were higher baseline RBF (4.3±0.4 versus 2.5±0.2 mL/min per gram; P P PHOX , a subunit of NAD(P)H oxidase, plays a regulatory role in the maintenance of renal vascular tone. These results also suggest that the mechanism of Ang II-mediated renal vascular action involves concomitant generation of O 2 − .Keywords
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